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Traditionally, it was assumed that ATP synthesis in mitochondria would be via high-energy phosphate group transfer intermediates, but none have ever been found. How does the chemiosmotic theory explain their absence?

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According to the chemiosmotic theory, th...

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The potential energy stored in the proton gradient that drives oxidative phosphorylation is totally chemical in nature.

A) True
B) False

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The reduction of atmospheric N2 to NH3 by certain species of bacteria is referred to as _______.

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A nucleotide is a


A) base found in nucleic acids.
B) purine or pyrimidine base plus a five-carbon sugar.
C) purine or pyrimidine base, a five-carbon sugar, and one or more phosphate groups.
D) purine or pyrimidine base, a five-carbon sugar, and three phosphate groups.

E) A) and D)
F) A) and C)

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Where in "typical" eukaryotic cells does the citric acid cycle occur? Be specific if the process or event occurs in only one part of an organelle. If you are not sure, give the location of the enzymes that catalyze the process.

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The mitoch...

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Fatty acids are broken down in a stepwise process, _______ carbon(s) at a time.


A) one
B) two
C) four
D) six

E) C) and D)
F) B) and C)

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What is the definition of Gibbs free energy (G)?

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G is the free energy of a reaction (in o...

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In plants, the molecule that captures energy from sunlight is


A) NADPH.
B) chlorophyll.
C) chloroplast.
D) chromatin.

E) A) and C)
F) A) and B)

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The production of one glucose from two pyruvates requires _______ more than is obtained through glycolysis.


A) 2 NADH
B) 2 ATP and 2 GTP
C) 2 NADH and 2 ATP
D) 4 ATP

E) A) and C)
F) A) and D)

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What is the definition of free-energy change?

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Free-energy change is the ener...

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Because fatty acids are synthesized by the stepwise addition of acetyl CoA derivatives, they differ in length by units of _______ carbon(s) .


A) 1
B) 2
C) 3
D) 4

E) A) and C)
F) B) and D)

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In photosynthesis, energy from sunlight is used to drive the synthesis of ATP and NADPH.

A) True
B) False

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Antimetabolite chemotherapy succeeds as a treatment for childhood leukemia because


A) cancer cells are dividing rapidly.
B) cancer cells are qualitatively different from normal cells.
C) normal cells are totally unaffected by the antimetabolite.
D) normal cells are impermeable to the antimetabolite.

E) B) and D)
F) B) and C)

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The _______ produced by the electron transport chain drives a flow of these ions through a complex of proteins called _______ to produce ATP from ADP and Pi.

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proton gradient (ele...

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Nucleic acid synthesis occurs by polymerization of nucleotide monophosphates into DNA or RNA.

A) True
B) False

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In humans, how many amino acids must be taken up in the diet?


A) 0
B) 9
C) 19
D) 20

E) B) and D)
F) All of the above

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What is the change in free energy ( Ξ”\Delta G) for the hydrolysis of ATP to ADP and Pi in the cell?


A) +12 kcal/mol
B) -1.2 kcal/mol
C) -7.3 kcal/mol
D) -12 kcal/mol

E) None of the above
F) A) and D)

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The conversion of A into B has a Ξ”\Delta G of +13 kcal/mol, and the conversion of C into D has a Ξ”\Delta G of -25 kcal/mol. If the two reactions are coupled through an enzyme, such that A + C goes to B + D, what is the free energy change, and in which direction will the reaction proceed?

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When two reactions are coupled, the chan...

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Suppose that in a cell culture experiment you add glucose uniformly labeled with carbon-14 to the culture medium. You then assay for the kinetics of carbon-14 incorporation into various amino acids. You find that carbon-14 is incorporated into histidine before it is incorporated into aspartate. Why?

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Amino acids derive their carbon skeleton...

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Gluconeogenesis involves the conversion of pyruvate to glucose. In a formal sense, this is essentially the reversal of glycolysis. However, the actual reactions are different. Explain this.

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Glycolysis is not an equilibrium set of ...

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